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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Mol Psychiatry. 2015 Jan 6;20(11):1373–1385. doi: 10.1038/mp.2014.166

Figure 3.

Figure 3

Neither DA nor SKF 83959 induces calcium mobilization in stable cell lines expressing D1 and/or D2 receptors. Using the FLIPR5 calcium assay system, intracellular calcium levels were measured every 2 seconds and plotted against time. Ligands (ACh = 10 µM acetylcholine, vehicle, DA = 10 µM dopamine, 10 µM SKF83959, 10 µM U73122, or 10 µM pirenzepine) were added at 20 secs, indicated by an arrow, in (a) D1R, (b) D2R, and (c) D1R/D2R stable cells. Calcium level is shown in percentage normalized to 10 µM acetylcholine (ACh). Traces are representatives of n = 3 experiments.