Fig 1. Setup of the low-cost and rapid TTC.

Major components include two 16 oz thermoses, a pan-and-tilt servo set to control the up and down and rotational motion to shuttle PCR vessels in and out of the thermoses. Also included are the battery pack, the Arduino electronic controller, and a breadboard. To reduce cost, the pan-and-tilt setup is constructed using a soup can, a wood stick, and a PCR tube holder made by metal wire. The parts were glued together using removable tape.