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. 2015 Jul 6;10(7):e0131701. doi: 10.1371/journal.pone.0131701

Fig 8. Speed and sensitivity of PCR reactions demonstrated by TTC.

Fig 8

PCR reactions to amplify 281 bp of the nuc gene from 2000, 200, 20, or 2 copies of S. aureus genomic DNA. (A) Lanes 1 to 4: PCR products from commercial thermal cycler. Lane 5: ladder. Lanes 6 to 9: PCR products from TTC with thin-walled plastic tubes, using a protocol of 2 min hot-start, followed by 40 cycles of 11 s and 17 s denaturation and annealing/extension. The 40-cycle reactions were completed in 22 min. (B) Lanes 1 to 4: PCR products from commercial thermal cycler. Lane 5: ladder. Lanes 6 to 9: PCR products from TTC with glass capillary tubes, using a protocol of 2 min hot-start, followed by 40 cycles of 7 s and 8 s denaturation and annealing/extension. The reactions were completed in 13 min and 3 s.