Fig 5. A2BR agonist does not directly stimulate γδ T cells.

A and B) γδ T cells isolated from immunized B6 mice at day 13 post-immunization were cultured in the presence or absence of A2BR agonist for 48 h. then IL-17 levels in the supernatants were measured by ELISA (A) and expression of IL-17 and CD44 on the cells was examined by staining and FACS analysis (B). C) Adenosine binding assay showing that γδ T cells do not bind adenosine via A2BR. In 96-well plates, γδ T cells (1x10⁵/well) isolated from IRBP1-20-immunized mice at day 13 post-immunization were incubated for 30 min with vehicle (Control) or an antagonist of the A1AR (DPCPX, 50 nM), A2AR (SCH 58261, 100 nM), A2BR (MRS 1754, 100 nM), or A3AR (MRS1220, 5 μM), then for 1 h with radiolabeled adenosine (³H-adenosine, 100 nM). The amount of labeled adenosine bound was measured **P<0.01.