Table 1. Yeast strains used in this study.
Strain | Genotype | Source |
---|---|---|
WT (BY4741) | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | Euroscarf |
WT (BY4743) | MATa/MATα his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 met15Δ0/MET15 LYS2/lys2Δ0 ura3Δ0/ura3Δ0 | Euroscarf |
sub1Δ::kanMX4 (BY4741) | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 sub1Δ::kanMX4 | Euroscarf |
sub1Δ::kanMX4 (BY4742) | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 sub1Δ::kanMX4 | Euroscarf |
sub1Δ::kanMX4 (S288c) | MATa/MATα his3Δ1/his3Δ1 leu2Δ0/leu2Δ 0 met15Δ0/MET15 LYS2/lys2Δ0 ura3Δ0/ura3Δ0 sub1Δ::kanMX4/sub1Δ::kanMX4 | This study |
NKY3822 (SK1) | MATa/MATα ho::hisG/ ho::hisG leu2::hisG/ leu2::hisG ura3 (Δsma-pst)/ ura3 (Δsma-pst) | Nancy Kleckner |
sub1Δ::natMX4 (SK1) | MATa/MATα ho::hisG/ ho::hisG leu2::hisG/ leu2::hisG ura3 (Δsma-pst)/ ura3 (Δsma-pst) sub1Δ::natMX4/ sub1Δ::natMX4 | This study |
WT SUB1-TAP (SK1) | MATa/MATα ho::hisG/ ho::hisG leu2::hisG/ leu2::hisG ura3 (Δsma-pst)/ ura3 (Δsma-pst) SUB1::TAP-KanMX4/ SUB1::TAP-KanMX4 | This study |