Table 2. Plasmids used in this study.
| Plasmid | Description | Source/reference |
|---|---|---|
| pAG25 | PAgTEF1-natMX4-TAgTEF1 | 18 |
| pYM13 | TAP-kanMX4 | 19 |
| pPS189 | PTEF2 in pRS426 URA3 | 20 |
| pRV765 | SUB1 ORF in pPS189 | This study |
| pRV920 | sub1(Y66A) allele in pPS189 | This study |
| pRV917 | PC4 cDNA in pPS189 | This study |
| pPS887 | GFP under PTEF2 in pRS416 URA3 | This study |
| pRV888 | SUB1 ORF in pPS887 | This study |
| pRV921 | sub1(Y66A) allele in pPS887 | This study |
| pRV918 | PC4 cDNA in pPS887 | This study |
| pPS883 | SUB1 ORF-6XHis-HA-Protein A under PGAL1 in pBG1805 URA3 | Open Biosystems |