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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Nat Struct Mol Biol. 2015 Mar 2;22(4):319–327. doi: 10.1038/nsmb.2982

Figure 1. Lnc-pri-miR-122 transcripts are capped but not polyadenylated.

Figure 1

a. Gene map showing exon-intron structure and 3′ positioned pre-miR-122 hairpin. Position of primers and Northern probes are indicated. b. Northern blot showing spliced and unspliced lnc-pri-miR-122 from human liver and three cell lines as indicated. Levels of γ-actin mRNA and mature miR-122 were also measured. Note reduced levels of liver RNA were employed. c. Northern blot detecting unspliced and spliced lnc-pri-miR-122 in human liver RNA using intron and exon-specific probes. d. Levels of m7G-cap immunoselected RNA measured by RT-qPCR as indicated. e. Proportion of pA+ RNA relative to pA− for transcripts indicated measured by RT-qPCR, and for lnc-pri-miR-122 and γ-actin measured by northern blotting. Error bars represent s.d. of an average (n=3 independent experiments).