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. 2015 Jul 6;11(7):e1005301. doi: 10.1371/journal.pgen.1005301

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© 2015 Varas et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 7 — (A-F) FISH of metaphase I spreads of WT, Atfas1-4 Atrad51-3 and Atfas1-4 Atrad51-2 PMCs using telomeric (red) and centromeric (green) probes. (A) WT metaphase I. (B, B’) Magnification of one bivalent from (A). The external localization of the telomeres (red dots) shows a chiasmatic association (star) between two bioriented homologous chromosomes (centromeres are represented as green circles). Representative images of metaphase I cells from (C) Atfas1-4 Atrad51-3 and (E) Atfas1-4 Atrad51-2. (D, D’, F, F’) Bivalent magnification and diagrammatic chiasma interpretation. (G, H) CO confirmation (arrow) in Atfas1-4 Atrad51-2 detected by immunolocalization of AtMLH1 (red) and AtZYP1 (green) and counterstained with DAPI (blue). Bars = 5 µm.