Fig 5. RMS cell growth and signalling is downregulated by ALK inhibitors.

(A) Cell cycle analysis of RMS cells treated with the indicated doses of Crizotinib and TAE684 for 24 hours. Cell cycle phases distribution measured with FACS-Calibur Cell Cytometer and represented by stacked bar graphs. The percentage of cells in sub G1 phase is shown. (B) Lysates from ALK inhibitor-treated and untreated RMS cells, probed with antibodies directed against total and phosphorylated AKT and ERK proteins, as well as against full-length and cleaved PARP (arrowhead). γ-Tubulin was used as internal loading control.