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. 2015 Jul 6;10(7):e0131253. doi: 10.1371/journal.pone.0131253

Fig 3. Combined UVB and quercetin treatment enhances sub-G1 cell cycle arrest and cleavage of chromosomal DNA.

Fig 3

A, analysis of cell cycle progression in B16F10 cells treated with Qu and/or UVB. Cells were collected (including floating cells), stained with propidium iodide and analyzed for DNA content by BD FACS Calibur Aria. ‘Sub-G1 peak’ contains apoptotic cells. B, represents the fold increase in the percentage of sub-G1 cells relative to control. *, P<0.05; **, P<0.01 for control versus treated; #, P<0.05; ##, P<0.01 for UVB-alone treated versus UVB + Qu treated. C, analysis of DNA fragmentation in B16F10 cells at 24 h post-Qu treatment. D, analysis of DNA fragmentation in B16F10 cells at 24 h post-UVB/Qu treatment.