Fig 7. Effect of quercetin on major protein regulators of anti-oxidant defence response in UVB-irradiated B16F10 cells.
A, immunoblot analysis of Nrf-2, Catalase and Cu/Zn SOD in B16F10 cells treated with Qu and/or UVB. Signals were quantified for Nrf-2 (B), catalase (C) and Cu/Zn SOD (D) and normalized against β-actin of each band using the Image Lab Software. E, immunoblot analysis of whole cell, cytosolic and nuclear NF-κB in B16F10 cells treated with Qu and/or UVB. F, represents the densitometric analysis of NF-κB (whole cell). G, the signals for cytosolic and nuclear NF-κB were quantified and expressed as ratio of NF-κB(cytosol) / NF-κB(nucleus) for each treatment.