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. 2015 Jul 6;10(7):e0131785. doi: 10.1371/journal.pone.0131785

Fig 2. Dextran sulfate effectively reduces the non-specific interaction of CD31 aptamers.

Fig 2

(A) 293FT cells were incubated with various concentrations (0, 0.2, 2, 20, and 200 nM) of CD31 aptamer clone 1 (AT-1, Cy5-labeled) or control aptamers (FITC-labeled) and subjected to flow cytometry analysis (n = 5). (B) EPCs or 293FT cells were separately incubated with control aptamers (Ctrl AT, FITC-labeled) or CD31 aptamers (AT-1, Cy5-labeled, 200 nM) with or without 0.2 mM dextran sulfate and subjected to flow cytometry. The overlap of histograms from 0 and 0.2 mM dextran sulfate experiments is shown (n = 5). (C) The mixture of EPCs and 293FT cells was incubated with CD31 aptamers (AT-1, Cy5-labeled, 200 nM) and various concentrations (0, 0.2, 1, and 5 mM) of dextran sulfate, followed by flow cytometry analysis (n = 3). (D) EPCs were incubated with CD31 antibodies (FITC-labeled) alone, CD31 aptamers alone (AT-1, Cy5-labeled, 200 nM), or with both CD31 antibodies and CD31 aptamers and subjected to flow cytometry analysis. Two-dimensional plots are shown (n = 5).