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. 2015 Jul 6;10(7):e0132323. doi: 10.1371/journal.pone.0132323

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© 2015 Nossol et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — Western blot analyses and immunofluorescence were used to examine different proteins of the tight junctions (ZO-1, occludin, claudin-3 and claudin-4) and the cytoskeleton CK18 and actin). Results comparable to IPEC-1 were found in IPEC-J2, here a weak expression was found at day 2 of culture for all proteins analysed using Western blot. The tight junctions proteins ZO-1 and occludin showed a strong expression at the cell border, however in this cell type ZO-1 had a much higher immunoreactivity as occludin. No actin-stress fibres were found in IPEC-J2. Claudin-3 and claudin-4 were present, but claudin-4 showed higher expression in the cytoplasm than at the cell border in comparison to claudin-3. (blue = DAPI; bar = 20 μm)