Table 1. qPCR—used primer sequences.

name	function	sequence 5‘ → 3‘ primer left	sequence 5‘ → 3‘ primer right	efficiency [%]	temperature [°C]
GAPDH	glycerinaldehyde-3-phos-dehydrogenase	acccagaagactgtgga	ttgagctcagggatgacctt	101.8	59.5
actin	cytoskeleton	gatgagattggcatggcttt	caccttcaccgttccagttt	99.3	58.3
VIL-1	Villin-1	caccatgaccaaactgaacg	tcgaagaagctgccataggt	98.4	54
MUC4	mucin-4	gctgacaggaagaggccata	ccccttcaactctggtgtgt	100	54
TLR4	Toll-like-rezeptor 4	ctggagacgactcaagaagc	agtgaaggctgttgtatcatgc	97.4	60.6
OCLN	occludin (TJ)	tgacactctaggcaatcaggtc	gggcccaatttccttatctg	98.5	54
VIL-2	villin-2 (ezrin)	cagtcgacgaaatcctgtga	ggtgcaggtccaacaaggta	97.5	57.1
Espin	espin	agaagcggaaagaggaggag	acttagcgatgtcccccttc	97.2	61.6
HIF1a	hypoxia inducible factor	tgccagaacctcctgtaacc	atgtacgtggggaggagatg	94.8	54
p53	p53	cgaactggcatgaaaat	agaagggacaaaggacgaca	105.8	56.2
CLDN1	claudin-1	ccagtgaagagagcctgacc	tgatgaggtgcagaagatgc	98.5	60.6
CLDN3	claudin-3	gtccatgggcctggagat	gatctgcgctgtgataatgc	99.5	58.7
CLDN7	claudin-7	ctcactcccaggacaagagc	tttgtgcgttgatagcttgc	99.3	60.6
BAD	BCL-2-antagonist	cttgcaaaaagagccgtttc	ttaagggcggaaaaacttca	98.3	57.6
BCL-2	apoptose-regulator	atttagcctcttgcctgtgg	gcagtttgaggctgcttttt	96.9	54
BAX	apoptose-regulator	ggtcgcgcttttctactttg	cgatctcgaaggaagtccag	99.6	60.6
PDH	pyruvat-dehydrogenase	acccgatcatgcttctcaag	tagcaaactgtgcagcatcc	96.7	54
SDH	succinat-dehydrogenase	actggatgggctgtacgagt	gtcgatcatccagcgatagg	96.7	55.7
COX5B	Cytochrome c oxidase subunit 5B	ggagagggaggtcatgatgg	ccactatccgcttgttggtg	100.2	61.2
18S	Ribosomal RNA	gcaattattccccatgaacg	ggcctcactaaaccatccaa	103.9	56.5
RPL10a	Ribosomal protein	tgcctttttggcttcagaat	actttggccaccatgttctc	98.7	54.7
Cyc 1	Cytochrome C	ctaccatgtcccaggtagcc	aaagcaagcccatcatcatc	96.7	54.7