Table 3. Comparison of microarray data and qPCR.
Important genes of the significantly regulated pathways shown in Table 2 were analysed via qPCR. The microarry data are shown and two reference genes were used to illustrate significant differences between both cell lines. Asterisks indicate significant differences from IPEC-1.
| Microarray analyses | reference gene 18S | reference gene beta-actin | |
|---|---|---|---|
| IPEC-J2 (IPEC-1 ≙ 1) | IPEC-J2 (IPEC-1 ≙ 1) | IPEC-J2 (IPEC-1 ≙ 1) | |
| PDH | 0.7 *** | 0.67** | 0.6*** |
| SDH | 1.4*** | 1.45*** | 1.01 |
| claudin 3 | 1.4 | 17.71*** | 14.66*** |
| Villin2 | 4.6*** | 4.11*** | 3.41*** |
| Villin1 | --- | 82.90*** | 70.52*** |
| Cyc1 | 0.8* | 0.71** | 0.62*** |
| TLR4 | 5.7* | 7.46*** | 6.76*** |
| Muc4 | 3.5* | 2.27** | 1.85* |
| p53 | 27.04 ** | 357.94*** | 278.4*** |
| BAD | 1.79*** | 1.97*** | 1.59*** |
| BAX | 2.23** | 1.58*** | 1.27 |
| BCL-X | --- | 1.13 | 0.94 |
| RPL10A | 0.9* | 0.79* | 0.7*** |
| Claudin-1 | 0.7* | 0.73** | 0.62** |
| Claudin-7 | 2.1** | 2.17*** | 1.93*** |
| HIF1a | 1.2* | 1.29* | 1.01 |
| COX5B | 1.23* | 1.08 | 0.86 |
| Occludin | 0.6*** | 0.50*** | 0.39*** |
| ESPN | 2.5* | 3.14*** | 2.46*** |
| 18S | --- | --- | 0.89 |
| beta-actin | --- | 1.12 | --- |
*p≤0.05;
**p≤0.01;
***p≤0.001