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. 2015 Jul 6;10(7):e0130000. doi: 10.1371/journal.pone.0130000

Fig 2. An internal deletion mutant stabilized Rev1 protein in the S phase.

Fig 2

A, Schematic diagram of the fission yeast Rev1 domain structure. Parallelogram, BRCT; rectangle, Y family-conserved region; ellipse, ubiquitin binding motif; hexagon, Lys-rich region. Rev1dK and Rev1dKK are internal deletion mutants lacking the amino acids from 761 to 818 and 761 to 797, respectively. B, The Rev1dKK mutant stabilized Rev1 protein. Flag-tagged rev1wt and rev1dKK cells were grown, and whole cell extracts were prepared by the boiling method. The upper panel shows a western blot of Rev1 protein, and the lower panel shows CBB staining of the membrane as a loading control. The left lane represents Rev1wt, and the right lane represents Rev1dKK. C, Rev1dKK protein was stable in S phase. Time course samples of the rev1dKKflag cdc25 strain were prepared. The samples were taken every 30 min after the release. The lower panels show the expression patterns of Rev1dKK, Cdc13, and Cdc2.