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. 2015 Jul 6;10(7):e0130000. doi: 10.1371/journal.pone.0130000

Fig 6. Increased expression of Rev1 sensitized the cells to cisplatin.

Fig 6

A, rev1dK exhibited cisplatin (CDDP) sensitivity. wt, rev1Δ, and rev1dK strains were grown. Cells were spotted on YES plates containing cisplatin with 5-fold serial dilutions, and the plates were incubated at 30°C for 3 days. The panels represent the growth of wt, rev1Δ, and rev1dK strains on cisplatin minus control and on a YES plate containing 100 μM cisplatin. B, Overexpression of rev1 conferred sensitivity to cisplatin. Cells harboring pREP41, pREP41-rev1, or pREP41-rev1dK plasmids were grown in EMM media without thiamine and spotted on an EMM plate containing 0 or 25μM CDDP with 5-fold serial dilutions. The plates were incubated at 30°C for 3 days. The panels represent the growth on an EMM plate without CDDP or containing 25 μM CDDP.