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. 2015 Jul 6;10(7):e0131676. doi: 10.1371/journal.pone.0131676

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© 2015 Stockdale et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — (A) Few tails, but no capsids, produced by mutant Portal_TP901-1::Ter. (B) Capsids, without attached tails, visualised for mutant MCP1_TP901-1::Ter. (C) Full, but scarce, phage particles produced following mutation MCP2_TP901-1::Ter. (D) Tails, but no capsids, produced for mutant Sfp_TP901-1::Ter. (E) Tails-only produced for mutant MHP_TP901-1::Ter. (F) Mature-resembling phages produced for mutant MCP3_TP901-1::Ter. (G) Unconnected capsids and tails observed in HTC1_TP901-1::Ter. (H) Distinct capsids and tails observed in HTC2_TP901-1::Ter. (I) Separated capsids and tails imaged from mutant Tap_TP901-1::Ter. (J) Capsids with long polytails isolated for mutant Ttp_TP901-1::Ter. (K) Capsids only are observed for mutant MTP_TP901-1::Ter. (L) Capsids and insoluble aggregates, proposed to be the TMP, observed in preparations of mutant gpG_TP901-1::Ter. (M) Capsids, but no tails, observed in preparations of mutant gpT_TP901-1::BamHI. (N) Mature-resembling phage produced by mutant gpGfsT_TP901-1. (O) Control TP901-1erm mature infectious phages. Refer to text for a more detailed description characterizing each mutant. Scale bars are 50 nm long.