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. 2015 Jul 6;10(7):e0130170. doi: 10.1371/journal.pone.0130170

Fig 6. Effect of the small molecules BAY11-7082 and ethyl pyruvate on NF-κB activity in yeast.

Fig 6

The strains containing the REs M2, RE4 and RelBCons were grown overnight (16 hours) in selective media containing low levels of galactose (0.008%) with or without the addition of different concentrations of BAY11-7082 (10μM, 20μM) and EP (1.5mM, 2.5mM, 5mM, 10mM). A-C) Average luciferase assays and standard errors of four biological repeats are presented. Results obtained with cells transformed with an empty expression vector are included to take into account the impact of the small molecules on the NF-κB-independent, basal expression of the reporter. D, E) Western blot images showing p50TAD, p50TAD + p65 and p65 protein levels in BAY and EP treated and untreated cells. PGK1 was used as a loading control.