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. 2015 Jun 5;25(7):801–817. doi: 10.1038/cr.2015.69

Figure 4.

Figure 4

AMOT specifically binds to Merlin. (A) Domain organizations of Merlin, AMOT-p130, and AMOT-p80. The interaction between Merlin and AMOT-CC is indicated by a two-way arrow. (B-F) Analytical gel filtration chromatography-based mapping of the minimal binding domains of AMOT and Merlin for their mutual interaction. The elution profiles of various forms of Merlin, AMOT, and their mixtures are shown in red, green, and black, respectively. (G) Analytical gel filtration chromatography showing that AMOT-CC does not bind to a Moesin fragment containing both the helical region and CTD. (H) Immunoprecipitation of overexpressed HA-tagged AMOT and endogenous Merlin showing that the AMOT/Merlin interaction occurs in cultured HEK293 cells, and this interaction is weakened by PAK1-mediated phosphorylation (right panel). The inputs are shown on the left. It is noted that in the AMOT-bound fraction of Merlin, no phosphor-Merlin is detected.