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. Author manuscript; available in PMC: 2015 Jul 7.
Published in final edited form as: Nat Protoc. 2011 Sep 29;6(10):1656–1668. doi: 10.1038/nprot.2011.402
Cell Number Total sonication Timing (Second) Conditions (Branson 250 digital)
10.000 90–115 0.7 sec. “ON”, 1.3 sec. “OFF”, 40 % Power
25.000 120–150 0.7 sec. “ON”, 1.3 sec. “OFF”, 40 % Power
50.000 150–180 0.7 sec. “ON”, 1.3 sec. “OFF”, 40 % Power
100.000 180–210 0.7 sec. “ON”, 1.3 sec. “OFF”, 40 % Power
500.000 210–240 0.7 sec. “ON”, 1.3 sec. “OFF”, 40 % Power