| Number of cycles | Temperature | Time |
|---|---|---|
| 1 | 98 °C | 1 min |
| 17 | 98 °C | 10 sec |
| 65 °C | 30 sec | |
| 72 °C | 30 sec | |
| 1 | 72 °C | 5 min |
| 1 | 4 °C | Until removed from the cycler |
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