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. Author manuscript; available in PMC: 2015 Jul 7.
Published in final edited form as: Nat Protoc. 2011 Sep 29;6(10):1656–1668. doi: 10.1038/nprot.2011.402
Cell Number Antibody concentration Bead Volume (bed volume)
10,000 0.25 μg per 1 ml 7–10 μl
25,000 0.4 μg per 1 ml 10 μl
500,000 1 μg per 1 ml 20 μl
10,000,000 10 μg per 10 ml 70 μl