Step 1 Reaction Mix | |
---|---|
5X Sequenase Buffer | 1 μl |
dNTP (3mM) | 1.5 μl |
DTT (0.1 M) | 0.75 μl |
BSA (10mg/ml) | 1.5 μl |
Sequenase Enzyme (13 U/μl) | 0.3 μl |
TOTAL Volume | 5.05 μl |
Step 1 Reaction Mix | |
---|---|
5X Sequenase Buffer | 1 μl |
dNTP (3mM) | 1.5 μl |
DTT (0.1 M) | 0.75 μl |
BSA (10mg/ml) | 1.5 μl |
Sequenase Enzyme (13 U/μl) | 0.3 μl |
TOTAL Volume | 5.05 μl |