| Step 1 Reaction Mix | |
|---|---|
| 5X Sequenase Buffer | 1 μl |
| dNTP (3mM) | 1.5 μl |
| DTT (0.1 M) | 0.75 μl |
| BSA (10mg/ml) | 1.5 μl |
| Sequenase Enzyme (13 U/μl) | 0.3 μl |
| TOTAL Volume | 5.05 μl |
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