| Step 2 PCR reaction mix | |
|---|---|
| Step 1 product (diluted) | 15 μl |
| GC reaction Buffer (5X) | 10 μl |
| dNTPs (10 mM) | 2 μl |
| Primer 2 (10 μM) | 1 μl |
| DMSO (100 %) | 1 μl |
| H2O | 20 μl |
| Phusion Enzyme (2 U/ μl) | 1 μl |
| TOTAL Volume | 50 μl |
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