Table 1.
Moonlighting paralogs in Saccharomyces cerevisiae.
| Moonlighting gene | Paraloga | % IDb | Conserved moonlightingc | Sourced | Function | Additional function(s) |
|---|---|---|---|---|---|---|
| RPS14A | RPS14B | 98 | Yes* | 1 | Component of the ribosome small subunit (40S) | Represses the expression of RPS14B |
| RPL2A | RPL2B | 100 | Yes* | 1 | Component of the ribosome large subunit (60S) | Regulates the accumulation of L2 mRNA |
| RPS28A | RPS28B | 99 | Yes*/** | 1 | Component of the ribosome small subunit (40S) | Shortens the half-life of its own mRNA |
| HXK2 | HXK1 | 77 | Yes (see text) | 1,2 | Hexokinase | Transcriptional regulator |
| ENO1 | ENO2 | 95 | Yes (see text) | 1,2 | Enolase | Required for vacuole homotypic membrane fusion and protein trafficking to the vacuole. Involved in tRNA mitochondrial targeting |
| ENO2 | ENO1 | 95 | Yes (see text) | 1 | Enolase | Required for vacuole homotypic membrane fusion and protein trafficking to the vacuole. Involved in tRNA mitochondrial targeting |
| CYC1 | CYC7 | 84 | No | 1 | Component of the mitochondrial electron-transport chain | Promotes apoptosis |
| SIS2 | VHS3 | 63 | Yes (see text) | 1,2 | Subunit of phosphopantothenoylcysteine decarboxylase (PPCDC) | Inhibitory subunit of protein phosphatase Ppz1 |
| VHS3 | SIS2 | 63 | Yes (see text) | 1,2 | Subunit of phosphopantothenoylcysteine decarboxylase (PPCDC) | Inhibitory subunit of protein phosphatase Ppz1 |
| TSA1 | TSA2 | 86 | Yes** | 1,2 | Peroxiredoxin peroxidase | Molecular chaperone |
| TSA2 | TSA1 | 86 | Yes** | 1 | Peroxiredoxin peroxidase | Molecular chaperone |
| CCM1 | PET309 | 27 | No | 2 | Supports bI4 maturase activity (removal of COB and COX1 fourth introns) | Key to maintain the steady-state levels of the mito-ribosome small subunit RNA |
| GAL1 | GAL3 | 74 | No (see text) | 3 | Galactokinase | Transcriptional regulator |
| LYS20 | LYS21 | 92 | Yes** | 4 | Homocitrate synthase | DNA-damage repair |
aNCBI blastp suite-2sequences.
bObtained from the Fungal Orthogroups Repository (http://www.broadinstitute.org/regev/orthogroups/).
c(*) Sequence conservation suggest that both copies retain the moonlighting behavoir; (**) Phenotypes of the paralog suggest that moonlighting activity is preserved.
d(1) Moonprot (Mani et al., 2015); (2) MultitaskProtDB (Hernandez et al., 2014); (3) Abramczyk et al., 2012; (4) Scott and Pillus, 2010.