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. 2015 Jun 29;212(7):1095–1108. doi: 10.1084/jem.20142110

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© 2015 Rahimpour et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — Tissue distribution of MAIT cells. (A) Flow cytometry analysis of naive mouse thymus, enriched thymus, spleen, lymph nodes (inguinal), liver, lung, and lamina propria, showing reactivity to MR1–5-OP-RU tetramer or MR1–Ac-6-FP tetramer. Plots depict lymphocytes with B220⁺ B cells excluded by electronic gating. B6 mice are depicted in the top group and BALB/c in the bottom group. Numbers indicate the percentage of MAIT cells (red gate) of total αβ T cells (black gate). (B) Scatter plots depict MAIT cells as a proportion of T lymphocytes in all tissues tested, gated as shown in A. Each symbol represents an individual mouse. Bars depict mean ± SEM, and data are derived from a minimum of three independent experiments for each strain, with a combined total of 6–12 mice per tissue.