Table 1.

TCR sequences of MR1–5-OP-RU tetramer⁺ TCR intermediate cells

Seq	TRAV	TRAJ	CDR3α	TRBV	TRBJ	CDR3β
1	TRAV1	TRAJ33	CAVRDSNYQLIW	TRBV13-2	TRBJ2-7	CASGDGDWGREQYF
2	TRAV1	TRAJ33	CAVRDSNYQLIW	TRBV13-3	TRBJ2-3	CASSRXGGXSAETLYF
3	TRAV1	TRAJ33	CAVRDSNYQLIW	TRBV13-3	TRBJ2-5	CASSDGTXQDTQYF
4	TRAV1	TRAJ33	CAVRDSNYQLIW	TRBV13-3	TRBJ1-3	CASSGTSSGNTLYF
5	TRAV1	TRAJ33	CAVRDSNYQLIW	TRBV13-2	TRBJ2-3	CASGGGDSAETLYF
6	TRAV1	TRAJ33	CAVRDSNYQLIW	TRBV13-3	TRBJ2-3	CASSDTGGAQETLYF
7	TRAV1	TRAJ33	CAVRDSNYQLIW	TRBV1	TRBJ2-4	CTCSGDSQNTLYF
8	TRAV1	TRAJ33	CAVRDSNYQLIW	TRBV13-2	TRBJ2-7	CASGVGDYEQYF
9	TRAV1	TRAJ33	CAVRDSNYQLIW	TRBV13-2	TRBJ2-7	CASGDGGAPSYEQYF
10	TRAV1	TRAJ33	CAVRDSNYQLIW	TRBV13-2	TRBJ2-3	CASGDDWGGRAETLYF
11	TRAV1	TRAJ33	CAVRDSNYQLIW	TRBV13-2	TRBJ2-5	CASGLGGRQDTQYF
12	TRAV1	TRAJ33	CAVRDSNYQLIW	TRBV13-3	TRBJ2-7	CASSAGTSSYEQYF
13	TRAV1	TRAJ33	CAVMDSNYQLIW	TRBV13-2	TRBJ2-3	CASGGDGDSAETLYF
14	TRAV1	TRAJ33	CAVRDSNYQLIW	TRBV13-3	TRBJ2-1	CASSKGDYAEQFF
15	TRAV1	TRAJ33	CAVRDSNYQLIW	TRBV13-2	TRBJ2-3	CASGEETGGAETLYF
16	TRAV1	TRAJ33	CAVRDSNYQLIW	TRBV13-3	TRBJ1-2	CASSGTTNSDYTF
17	a) TRAV1 b) TRAV6-4a	TRAJ33	CAVRDSNYQLIW	TRBV13-2	TRBJ2-5	CASGLGGRQDTQYF
18	a) TRAV1 b) TRAV6-5a	TRAJ33	CAVRDSNYQLIW	TRBV13-2	TRBJ2-3	CASGDADRGQGAETLYF
19	a) TRAV1 b) TRAV3-3a	TRAJ33	CAVRDSNYQLIW	TRBV13-3	TRBJ2-4	CASSDAASQNTLYF
20	a) TRAV1 b) TRAV9Na	TRAJ33	CAVRDSNYQLIW	TRBV13-2	TRBJ2-1	CASGDGDSYAEQFF
21	a) TRAV1 b) TRAV12a	TRAJ33	CAVRDSNYQLIW	TRBV13-2	TRBV2-7	CXSGDGGAPSYEQYF

MR1–5-OP-RU tetramer⁺ TCR-β intermediate cells from B6 mouse spleen, gated in a similar manner to that shown in Fig. 1 C, were sorted as single cells, and TCR-α and -β chains were sequenced. Data are derived from two independent experiments with spleens from two mice per experiment. X, sequence unclear.

^aMixed unproductive sequences.