Table 2.

TCR sequences of MR1–5-OP-RU tetramer⁺ cells in PLZF KO mice

Seq	TRAV	TRAJ	CDR3α	TRBV	TRBJ	CDR3β
1	TRAV1	TRAJ33	CAVRDSNYQLIW	TRBV13-2	TRBJ2-1	CASGDAEDYAEQFF
2	TRAV1	TRAJ33	CAVRDSNYQLIW	TRBV13-3	TRBJ2-4	CASSDRGXGSQNTLYF
3	TRAV1	TRAJ33	CAVRDSNYQLIW	TRBV13-3	TRBJ2-7	CASGDAEDYAEQFF
4	TRAV1	TRAJ33	CAVRDSNYQLIW	TRBV19	TRBJ2-7	CASSIGDTYEQYF
5	TRAV1	TRAJ33	CAVRDSNYQLIW	TRBV19	TRBJ2	CASSPTGGEGYAEQFF
6	TRAV1	TRAJ33	CAVRDSNYQLIW	TRBV13-3	TRBJ1-2	CASGDGDSYAEQFF
7	a) TRAV1 b) TRAV6a	TRAJ33	CAVRDSNYQLIW	TRBV13-3	TRBJ2-2	CASSEGRDTGQLYF
8	TRAV1	TRAJ33	CAVRDSNYQLIW	TRBV14	TRBJ2-7	CASSFDIYEQYF
9	TRAV1	TRAJ33	CAVRDSNYQLIW	TRBV13-3	TRBJ2-1	CASSGTVYAEQFF
10	TRAV1	TRAJ33	CAVRDSNYQLIW	TRBV13-2	TRBJ2-1	CASGGTYYAEQFF
11	TRAV1	TRAJ33	CAVRDSNYQLIW	TRBV13-3	TRBJ2-7	CASTGTGISYEQYF
12	TRAV1	TRAJ33	CAVRDSNYQLIW	ND
13	TRAV1	TRAJ33	CAVRDSNYQLIW	TRBV13-3	TRBJ2-4	CASXXXGGASQNTLYF
14	a) TRAV1 b) TRAV5Da	TRAJ33	CAVRDSNYQLIW	TRBV13-3	TRBJ2-5	CASSDRGQDTQYF
15	a) TRAV1 b) TRAV10a	TRAJ33	CAVRDSNYQLIW	TRBV13-2	TRBJ2-1	CASGDGGAPSYEQYF
16	a) TRAV1 b) TRAV10a	TRAJ33	CAVRDSNYQLIW	TRBV1	TRBJ2-4	CTCSAVWFSQNTLYF

MR1–5-OP-RU tetramer⁺ TCR intermediate cells from PLZF^null mouse spleen, gated in a similar manner to that shown in Fig. 4 C, were sorted as single cells, and TCR-α and -β chains were sequenced. Cells were derived from spleens from two mice per group. X, sequence unclear.

^aMixed unproductive sequences.