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. 2015 Jun 29;212(7):1001–1009. doi: 10.1084/jem.20150191

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© 2015 Good-Jacobson et al.

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Figure 5. — c-Myb regulates migration to CXCL12. (A and B) Cd23^Cre/+ (dotted gray line or closed squares) and c-Myb^fl/flCd23^Cre/+ (black solid line or open circles) mice were immunized with NP-KLH precipitated in alum and spleens harvested at day 7 after immunization. (A) Splenic plasmablasts (B220^loCD138^hi) were assessed for CXCR4 expression, representative of plasmablasts assessed at multiple time points after immunization. (B–D) CD138-enriched splenic cells were assessed for the ability of plasmablasts to migrate to CXCL12 (B) representative plot, (C) combined data from three independent experiments, and (D) CXCL10, combined data from two independent experiments. *, P < 0.05 (Mann-Whitney nonparametric, two-tailed test; mean ± SEM). (E–G) Cd23^Cre/+ and c-Myb^fl/flCd23^Cre/+ mice were immunized with NP-KLH precipitated in alum and spleens harvested at day 7 after immunization. Sections were stained with B220 (blue) and IgG1 (red; E), and CD3 (blue) and IgG1 (red; F), representative of three spleens per genotype; ASCs in T cell zones were enumerated (G; n ≥ 7 per genotype of T cell zones assessed). Bars, 200 µm. *, P < 0.05 (unpaired two-tailed t test; mean ± SEM).