Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jun 29;212(7):1061–1080. doi: 10.1084/jem.20141601

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Lee et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 6. — VPA treatment further accelerates cutaneous wound healing in CXXC5^−/− mice. After generation of full-thickness wounds (diameter = 1.5 cm) on the backs of CXXC5^+/+ and CXXC5^−/− mice, 500 mM VPA was applied topically to the wounds daily (n = 10 mice/group). (A) Representative images of macroscopic wounds, H&E, and IHC staining showing expression of β-catenin, CXXC5, keratin 14, collagen I, and PCNA in the wounds of CXXC5^+/+ and CXXC5^−/− mice treated with or without VPA treatment 12 d after wounding (n = 4 mice/group) are shown (n = 3 independent experiments). Dashed lines indicate the epidermal–dermal boundary. F, fibroblasts; K, keratinocytes. (B) Relative wound closure rates describe the effects of VPA on wound healing in CXXC5^+/+ and CXXC5^−/− mice. Wound sizes were measured at 1, 3, 5, 7, 9, and 12 d after wounding (**, P < 0.005; ***, P < 0.0005; n = 10 mice/group). (C) Western blot analyses of β-catenin, CXXC5, keratin 14, α-SMA, collagen I, PCNA, endothelin-1, c-Myc, cyclin D1, and Erk were performed in wounds of CXXC5^+/+ and CXXC5^−/− mice treated with or without VPA (n = 2 mice/group) 12 d after wounding (n = 2 independent experiments). Relative densitometry values are shown below blots. (D) Representative images of Masson’s trichrome, picrosirius red, or van Gieson staining of wounds of CXXC5^+/+ and CXXC5^−/− mice treated with or without VPA 12 d after wounding (n = 4 mice/group) are shown (n = 3 independent experiments). (A and D) Bars, 100 µm. (E) Hydroxyproline levels in wounds of CXXC5^+/+ and CXXC5^−/− mice treated with or without VPA 12 d after wounding are shown (*, P < 0.05; **, P < 0.005; ***, P < 0.0005; n = 4 mice/group). (F) Quantitative TissueFAXS analyses of IHC staining shown in A were performed in keratinocytes (left) and fibroblasts (right; *, P < 0.05; **, P < 0.005; ***, P < 0.0005; n = 3 independent experiments). Means ± SD.