Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jun 29;212(7):1021–1041. doi: 10.1084/jem.20150354

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Watson et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 6. — Genetic ablation of endothelial sAC inhibits leukocyte transmigration. (a) Quantitative PBMC TEM assays were performed on HUVECs expressing either scrambled (SCR) or sAC shRNA. Where indicated, the rescue construct (sAC_t) was also expressed. (b) Immunoblot analysis for sAC and β-actin was performed on samples treated in parallel. (c) Quantification of immunoblots. Total sAC expression was normalized to β-actin for each sample and then normalized to SCR control. (d and e) TEM-IF assays were performed on HUVECs expressing SCR, sAC, or CD99 shRNA. (f) Immunoblot analysis for CD99, sAC, and β-actin was performed on samples treated in parallel. (g) Quantification of immunoblots. Total sAC or CD99 expression was normalized to β-actin for each sample and then normalized to SCR control. (h) Quantitative PMN TEM-IF assays were performed on TNF-activated (4 h) HUVECs expressing SCR, sAC, or CD99 shRNA. (i) Quantitative PMBC TEM-IF assays were performed on TNF-activated (4 h) HUVECs expressing SCR, sAC, or CD99 shRNA. (j) Immunoblot analysis of samples treated in parallel were performed for sAC, ICAM-1, PECAM, JAM-A, and CD99 expression. Bars, 10 µm. Images are representative of three (b, e, and j) independent experiments. Numerical values are the mean of two (h and i) or three (a, c, d, and g) independent experiments. Error bars represent SD (c and g) or SEM (a, d, h, and i; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; Student’s t test [a and c] and ANOVA [d, g, h, and i]).