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Cycloheximide chase experiments with substrates of the Asi E3 ubiquitin ligase.
Degradation of 3HA-tagged proteins after blocking translation with cycloheximide. Whole cell extracts were separated by SDS-PAGE followed by immunoblotting with antibodies against the HA tag and Pgk1 as loading control. Representative immunoblots from 2 technical replicates. Left panel wild type and asi1Δ immunoblots are reproduced in Fig. 3f.
