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. 2015 Apr 3;4(2):157–181. doi: 10.3390/pathogens4020157

Scheme 2.

Scheme 2

Plasmid selection and maintenance by Operator Repressor Titration. Note of a: An essential gene is repressed by a genome encoded repressor. Plasmid containing several sequences of the operator can titrate this repressor by competition, allowing essential gene expression. GoI: Gene of Interest; ori: Origin of replication.