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. 2015 May 13;4(2):210–228. doi: 10.3390/pathogens4020210

Figure 6.

Figure 6

Electrophoretic analysis of S. marcescens 81 cell-free culture supernatants. Panel A. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) (12%) analysis of cell-free culture supernatant of S. marcescens 81 strain. Cell-free culture supernatant was concentrated and adjusted to 30 µL per line. Panel B. SDS-PAGE analysis of the Sm81 50 kDa protein purified by HiTrap Q FF ion exchange column. Twenty µg of Sm81P50 was charged in the gel line. Panel C. Zymography showing the 50 kDa protease from the S. marcescens strains cell-free culture supernatants. Gelatin-SDS-PAGE stained with coomassie brilliant blue. The protein concentration was adjusted to 6 µg per line. For Sm65 the protein concentration was 0.6 µg per line.