Table 4.

Summary of Experimental Details.

Experiment	Inoculation/Stagnation Periods	Dates Performed	Water Formulation; Temperature; Water Change Rate
A1	23 April 2010 Inoculation with 10 mL from Blacksburg hot water tank Incubate 1 week at 37 °C	5/18/10–8/3/10	Norton et al., 2004; 37 °C; 3 water changes per week
	5 May 2010 Inoculation with A. polyphaga Incubate 1 week at 37 °C
	12 May 2010 Inoculation with L. pneumophila and M. avium Incubate 1 week at 37 °C
	18 May 2010 End stagnation period
A2	17 September 2010 Inoculation with A. polyphaga Incubate 1 week at 37 °C	10/13/10–3/7/11 (5 months)	• 90% Norton et al., 2004/ 10% GAC filtered Blacksburg, VA tap water; 32 °C; 3 water changes per week
	27 Septermber 2010 Inoculation with L. pneumophila and M. avium Incubate 1 week at 37 °C
	13 October 2010 End stagnation period
A3	18 March 2010 Inoculation of V. vermiformis Incubate at 37 °C	3/31/11–4/18/11 (3 weeks)	• 90% Norton et al., 2004/10% GAC filtered Blacksburg, VA tap water with addition of amino acid, Mg, Fe, Zn and pH decrease to 7.5; 32 °C; 1 water change per week
	21 March 2010 Inoculation of L. pneumophila Incubate at 37 °C
	31 March 2011 End stagnation period