Table 2.
Percent of fibroblastic cells in compressed 3-D collagen ECM. N = 3 experiments; Mean ± Standard Deviation.
| Culture media | 1 day of culture | 4 days of culture | ||||
|---|---|---|---|---|---|---|
| Control | NSC23766 | NSC + Y-27632 | Control | NSC23766 | NSC + Y-27632 | |
| Serum-Free | 15 ± 13% | 78 ± 25% * | 0 ± 0% ** | 8 ± 10% | 80 ± 19% * | 0 ± 0% ** |
| PDGF | 7 ± 12% | 58 ± 37% * | 0 ± 0% ** | 9 ± 10% | 85 ± 13% * | 0 ± 0% ** |
| IGF | 19 ± 2% | 57 ± 25% * | 0 ± 0% ** | 12 ± 11% | 81 ± 13% * | 0 ± 0% ** |
| FGF | 95 ± 8% | 100 ± 0% | 0 ± 0% ** | 79 ± 26% | 97 ± 6% * | 0 ± 0% ** |
| TGFβ1 | 100 ± 0% | 100 ± 0% | 0 ± 0% ** | 100 ± 0% | 100 ± 0% | 0 ± 0% ** |
* Significantly greater than Control (P < 0.05, two way ANOVA); ** Significantly less than NSC23766 (P < 0.05, two way ANOVA).