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. 2015 Jul 7;5:11771. doi: 10.1038/srep11771

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Copyright © 2015, Macmillan Publishers Limited

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(a) Alignment of the coding region of the GFP variants. The alignment was created using GeneDoc (http://www.nrbsc.org/gfx/genedoc/). (b) Schematic representation of GFP transgenes used in photoconversion experiments. Pr1b-SP, secretory signal peptide from the tobacco pathogenesis-related protein 1; EGFP, enhanced green fluorescent protein; L, linker (GGGS)₃; KDEL, endoplasmic reticulum retrieval signal peptide; Htt^Q103, exon 1 of human Htt^Q103 gene; Cytosolic mGFP4-T in tobacco cell culture and cytosolic S65T-GFP in Drosophila gut cells. In cytosolic mGFP4-T, V (valine) was replaced by A (alanine), and Q (glutamine) was replaced by R (arginine) relative to EGFP. In cytosolic S65T-GFP, L (leucine) was replaced by F (phenylalanine) relative to EGFP.