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. 2015 Jul 7;5:11742. doi: 10.1038/srep11742

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Copyright © 2015, Macmillan Publishers Limited

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(A) Scheme illustrating schedule of EB culture in both PKD2 wild type (WT) and PKD2 kinase-dead (KD) iPSCs. (B–E) qPCR analysis depicting expression levels of different germ layers markers: Mesoderm - Brachyury (Bry); Endoderm -FoxA2; Ectoderm -Pax6, -Tubb3. (F–G) qPCR analysis illustrating expression of hemangioblast and cardiovascular progenitor markers: c-kit, PDGFR. (H–I) qPCR of early cardiac markers: Tbx5 and Isl1. (J–L) qPCR analysis of vascular markers CD34, CD31 and vWF. (M–N) qPCR of myocardial markers Myl2a and Myh6. (O) Immunostaining of differentiating ESC cultures for CD31 at day 14. Genotypes are indicated in the figure. All experiments were performed n = 3 in replicates. Significances were calculated using R. Raw p values were adjusted using Bonferroni correction (^§p < 0.05; ^§§p < 0.01; ^§§§p < 0.001). Adjusted p-values are listed in Suppl. Table 3.