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. 2015 Jul 7;6:499. doi: 10.3389/fpls.2015.00499

FIGURE 1.

FIGURE 1

Putative core betalain biosynthetic pathway in plants. S, spontaneous reaction; the primary enzymatic reactions are tyrosine hydroxylating activity (TyH), the enzyme for which has not been unequivocally determined; CYP76AD1, a cytochrome P450 enzyme that catalyzes oxidation of L-DOPA for the formation of cyclo-DOPA; and DOPA 4,5-dioxygenase (DOD) catalyzing the cleavage of L-DOPA needed for the formation of betalamic acid. Betacyanins normally accumulate as glycosides and may also be acylated. Regarding glucosylation at the 5-O-position, it appears that it is primarily done by cyclo-DOPA 5-O-glucosyltransferase (5GT) prior to the conjugation that forms the betacyanin.