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. 2015 Jul 7;6:499. doi: 10.3389/fpls.2015.00499

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Copyright © 2015 Chung, Schwinn, Ngo, Lewis, Massey, Calcott, Crowhurst, Joyce, Gould, Davies and Harrison.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Differential activity of PmDOD and PmDOD-like in a transient expression assay. White petal tissue of A. majus was bombarded with constructs for the genes (driven by the 35S promoter) followed by supplementation with DOPA or water. Representative petal samples are shown. A GFP construct was also included, except for the PmDOD-bombarded tissue in the magnified panels. Betalain pigments were observed only in PmDOD-bombarded tissue that was also fed DOPA and they had characteristic autofluorescence in blue light. No pigments were observed in PmDOD-like bombarded tissue despite GFP fluorescence indicating that the experimental conditions were sufficient for transformation. Scale bar = 300 μm.