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. 2015 Jul 7;6:499. doi: 10.3389/fpls.2015.00499

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Copyright © 2015 Chung, Schwinn, Ngo, Lewis, Massey, Calcott, Crowhurst, Joyce, Gould, Davies and Harrison.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Maximum likelihood analysis of LigB genes. Analysis used the nucleotide sequences of the putative ORFs. The Amaranthaceae species names are in red, species of other betalain producing Caryophyllales families in blue, an anthocyanin taxa within the Caryophyllales (Dianthus) in green, and the non-Caryophyllales species in black. Sequences for McDOD1 and McDOD2 are not full-length but span the catalytic domain. Bootstrap analysis used 1000 datasets, with results shown for nodes that have at least 70% support. The branch-lengths indicate the average number of substitutions per site, with the scale bar given at the bottom of the figure. See Supplementary Figure S4 for the accession numbers. The Class I grouping is indicated. For the Class II sequences, the published DOD of Portulaca grandiflora is indicated along with the activities of sequences assayed in this study.