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. 2015 Jul 7;5:11888. doi: 10.1038/srep11888

Figure 7. Intermediate monocytes produce increased amounts of IL-1β and IL-6 both basally and in response to stimulation with anti-MPO mAb.

Figure 7

CD14+ monocytes were isolated from PBMCs of healthy control individuals by MACS separation. Monocyte subsets were then sorted from CD14+ cells based on CD14 and CD16 expression. Sorted subsets of cells were incubated with 5 ng/ml TNF-α @ 37 °C for 30 minutes followed by stimulation for 4 hours with 5 μg/ml of either monoclonal antibody (mAb) directed against MPO or an isotype control. Supernatants were then removed and levels of (A) IL-1β, (B) IL-6 and (C) IL-8 measured by ELISA. Data are presented as the median and interquartile range of the fold increase over control. Statistical analysis was performed using Two-way ANOVA and Sidak test to correct for multiple comparisons (*p < 0.05, **p < 0.01, ***p < 0.001, ****p <0.0001). Class: Classical; Int: Intermediate; NC: Non-Classical