Figure 8. Fc receptor binding is not required for anti-MPO-induced IL-1β production by monocytes.

CD14+ monocytes were isolated from PBMCs of healthy control individuals by MACS separation. Cells were incubated with 2.5 μg/1 × 10⁶ cells Fc Block @ 37 ^°C for 30 minutes followed by stimulation for 4 hours with 5 μg/ml of either monoclonal antibody (mAb) directed against MPO or an isotype control. Supernatants were then removed and levels of IL-1β measured by ELISA. Data are presented as the median and interquartile range. Statistical analysis was performed using Two-way ANOVA and Sidak test to correct for multiple comparisons (**p < 0.01).