Table 2. Stability data of fructose-based 3-acetyl-2,3-dihydro-1,3,4-oxadiazole (GLB) detected by high performance liquid chromatography (HPLC) method in rat plasma (Mean ± SD, n = 3).

Stability	Actual concentration (μg/mL)	Measured concentration (μg/mL)	Precision (RSD, %)	Accuracy (RE, %)
Light and room temperature for 24 hours	0.025	0.026 ± 0.002	7.52	4.17
	0.5	0.486 ± 0.027	5.53	−2.79
	4	3.922 ± 0.055	1.41	−1.94
Post-preparative stability (at 4 °C for 48 hours)	0.025	0.026 ± 0.001	3.63	5.31
	0.5	0.528±0.007	1.25	5.62
	4	4.233 ± 0.196	4.64	5.83
Post-preparative stability (at 4 °C for 1 week)	0.025	0.026 ± 0.001	4.19	3.83
	0.5	0.428 ± 0.010	2.25	−14.4
	4	3.668 ± 0.219	5.98	−8.30
Freeze-thawing 3 cycles (at −20 °C)	0.025	0.025 ± 0.001	−0.75	2.82
	0.5	0.467 ± 0.036	−6.51	7.65
	4	3.732 ± 0.227	−6.71	6.08
Freezing storage stability (at −20 °C for 2 weeks)	0.025	0.027 ± 0.001	4.09	8.17
	0.5	0.535 ± 0.009	1.68	7.10
	4	4.393 ± 0.090	2.04	9.84
Freezing storage stability (at −20 °C for 1 month)	0.025	0.027 ± 0.002	8.29	8.66
	0.5	0.490 ± 0.015	3.01	1.17
	4	4.097 ± 0.213	5.19	−2.27
Freezing storage stability (at −20 °C for 3 months)	0.025	0.023 ± 0.002	−7.12	8.04
	0.5	0.487 ± 0.003	−2.66	−2.04
	4	3.918 ± 0.063	0.71	1.62