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. 2015 Jul 7;5:11955. doi: 10.1038/srep11955

Figure 3. RIPK4 depletion decreases cell proliferation and colony formation capability in SiHa and Caski cells in vitro.

Figure 3

(a, b) Real-time quantitative PCR analysis of RIPK4 mRNA expression and western blot analysis of RIPK4 protein expression in SiHa and Caski cells transfected with specific siRNAs targeting RIPK4 for 48 h. (c, d) Colony formation capacity of SiHa and Caski cells transfected with RIPK4 siRNAs and control siRNA were analyzed by colony formation assay. (e) Inhibition of SiHa cell proliferation by RIPK4 siRNAs tested by MTT assays. (f) Inhibition of Caski cell proliferation by RIPK4 siRNAs as measured by MTT assays. Values are the mean ± s.d. of three independent experiments. *P < 0.05 relative to the control.