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. 2015 Jun 30;3:e1065. doi: 10.7717/peerj.1065

Figure 1. EPR spectra of the C-terminal of SNAP-25 SN1 and SN2 in t-SNAREs binary complex under room temperature.

Figure 1

(A) The diagram of the spin-labeled SNAP-25 SN1 and SN2 with syntaxin 1A SNARE motif in t-SNAREs binary complex. The corresponding labeled positions of SNAP-25 SN1 are in red and that of SN2 are in blue. (B) EPR spectra of the spin-labeled C-terminal of SNAP-25 SN1 and SN2 in the full-length His-tag syntaxin 1A and spin-labeled GST-tag SNAP-25 binary complex. EPR spectra of the binary complex in the detergent and in the membrane are shown. The immobile and mobile components are indicated in red and blue arrows, respectively.