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. 2015 Jun 30;3:e1065. doi: 10.7717/peerj.1065

Figure 2. EPR spectra of the spin-labeled C-terminal of SNAP-25 SN2 in binary and ternary SNARE complexes.

Figure 2

(A) EPR spectra for three mutants at the C-terminal of SNAP-25 SN2 (177C, 184C and 198C) in solution. (B) EPR spectra for the same spin-labeled positions in membrane-bound syntaxin 1A and SNAP-25 binary complex. (C) Syntaxin 1A-SNAP-25-souble VAMP2 (1-94) ternary complex. All the binary and ternary complexes are prepared by His-tag syntaxin 1A pulling down spin-labeled GST-tag SNAP-25 mutants in the absence or presence of GST-tag soluble VAMP2.