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. 2015 Jun 30;3:e1065. doi: 10.7717/peerj.1065

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© 2015 Lu

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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The curves represent the lipid mixing when the wild-type or nitroxide spin-labeled SNAP-25 at positions of 181 and 188 were used in the proteoliposome fusion assay (protein/lipid ratio 1:200). The data were normalized against the maximum fluorescence intensity (MFI) obtained by adding 0.1% reduced Triton-X-100. The control runs with the v-SNARE vesicles and the t-SNARE vesicles without SNAP-25 (grey curve). The inset in the left corner is the electron micrograph of the SNAREs-reconstituted vesicles used in assays. The vesicles were stained with 1% phosphotungstic acid on the carbon grids. The size of the vesicles was 90 ± 15 nm.