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. 2015 Jun 30;3:e1065. doi: 10.7717/peerj.1065

Figure 4. The SDS-resistant ability of the truncated SNARE complex.

Figure 4

Four groups of different molecular ratio of one time full-length syntaxin 1A mixes with two times wild-type or truncated SNAP-25 and various soluble VAMP2 and runs the SDS-PAGE without boiling. The markers, from top to bottom, are 66, 45, 29 and 14.2 kDa. (A) Wild-type SNAP-25 with syntaxin 1A and soluble VAMP2, (B) BoNT/A truncated SNAP-25A (Δ9), (C) BoNT/E truncated SNAP-25E (Δ26). The red arrows indicate the position of the SNARE complex; the red brackets indicate the higher order of SNARE complexes due to SNAP-25 swapping.